Megaselia abdita

Megaselia abdita (syn. M. griseipennis(Phoridae)

The humpbacked fly Megaselia abdita is a perfect lab animal. The species is common in North America and Europe and is very easy to keep in the laboratory. The generation time of Megaselia abdita is about 3 weeks and its genome size about 653 Mb for the female and 553 Mb for the male (Spencer Johnston & Urs Schmidt-Ott, unpublished data). Besides its value as a model in evolutionary developmental biology, the species is used in forensic biology. Protocols for in situ hybridization, antibody staining, RNAi and mRNA injection in embryos have been established. For protocols return to the home page.

"Click to view basic protocols written by Schmidt-Ott lab"


Publications on Megaselia from the Schmidt-Ott lab:

- Wotton KR, Jiménez-Guri E, Crombach A, Janssens H, Alcaine-Colet A, Lemke S, Schmidt-Ott U, Jaeger, J. (2015). Quantitative system drift compensates for altered maternal inputs to the gap gene network of the scuttle fly Megaselia abdita. eLife 2015;10.7554/eLife.04785. PMID: 25560971

- Rafiqi Ab. M, Park CH, Kwan CW, Lemke S, Schmidt-Ott U (2012). BMP-dependent serosa and amnion specification in the scuttle fly Megaselia abdita. Development 139, 3373-3382.

- Rafiqi Ab. M, Lemke S, Schmidt-Ott U (2011). The scuttle fly Megaselia abdita (Phoridae): A link between Drosophila and mosquito development. Cold Spring Harb Protoc, doi:10.1101/pdb.emo143.

- Rafiqi Ab. M, Lemke S, Schmidt-Ott U (2010) Postgastrular zen expression is required to develop distinct amniotic and serosal epithelia in the scuttle fly Megaselia. Dev. Biol. 341: 282-290.

- Rafiqi Ab. M, Lemke SL, Ferguson S, Stauber M, Schmidt-Ott U (2008). Evolutionary origin of the amnioserosa in cyclorrhapahan flies correlates with spatial and temporal expression changes of zen. Proc. Natl. Acad. Sci. USA 105: 234-239.

- Lemke S, Stauber M, Shaw PJ, Rafiqi Ab. M, Prell A, Schmidt-Ott U. (2008). bicoid occurrence and Bicoid-dependent hunchback regulation in lower cyclorrhaphan flies. Evol. Dev. 10: 413-420.

- Stauber M, Lemke S, Schmidt-Ott U. (2008). Expression and regulation of caudal in the lower cyclorrhaphan fly Megaselia. Dev. Genes Evol. 218: 81-87.

- Bullock SL, Stauber, M, Prell A., Hughes J, Ish-Horowicz D, Schmidt-Ott U (2004). Differential Cytoplasmic mRNA Localization Adjusts Pair-Rule Transcription Factor Activity to Cytoarchitecture in Dipteran Evolution. Development 131, 4251-4261.

- Stauber M, Prell A, Schmidt-Ott U (2002) A single Hox3 gene with composite bicoid and zerknüllt expression characteristics in non-Cyclorrhaphan flies. Proc. Natl. Acad. Sci. USA 99, 274-279.

- Stauber M, Taubert H, Schmidt-Ott U (2000). Function of bicoid and hunchback homologs in the basal cyclorrhaphan fly Megaselia (Phoridae). Proc. Natl. Acad. Sci. USA 97, 10844-10849.

- Stauber M, Jäckle H, Schmidt-Ott U (1999). The anterior determinant bicoid of Drosophila is a derived Hox class 3 gene. Proc. Natl. Acad. Sci. USA 96, 3786-3789.

- Schmidt-Ott, U., González Gaitán, M., Jäckle, H., Technau, G. M. (1994). Number, identity and sequence of the Drosophila head segments. Proc. Natl. Acad. Sci. USA 91,8363-8367.

- Schmidt-Ott U, Sander K, Technau GM (1994). Expression of engrailed in embryos of a beetle and five dipteran species with special reference to the terminal regions. Roux's Arch. Dev. Biol. 203, 298-303.

See also:

- Wotton KR, Jiménez-Guri E, García Matheu B, Jaeger J (2014). A staging scheme for the development of the scuttle fly Megaselia abdita. PLoS One 9:e84421. doi: 10.1371/journal.pone.0084421.